TM4 Basal Medium Product ID: T7954
Introduction
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
2.99 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
T7954 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
320 |
| A113 |
Ammonium Phosphate |
230 |
| A305 |
Ammonium Sulfate |
134 |
| B210 |
Boric Acid |
|
| C266 |
Calcium Chloride, Anhydrous |
113.25 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
|
| E410 |
EDTA, Disodium Salt |
18.5 |
| F263 |
Ferrous Sulfate, Heptahydrate |
13.9 |
| M150 |
Magnesium Sulfate, Anhydrous |
|
| M250 |
Manganese Sulfate |
|
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| B140 |
D-Biotin |
0.05 |
| C232 |
Choline Chloride |
0.1 |
| F430 |
Folic Acid |
0.5 |
| G503 |
Glycine |
2.5 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
5.0 |
| P866 |
Pyridoxine HCl |
0.5 |
| T390 |
Thiamine HCl |
0.5 |
T7954 TM4 Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
2.99g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Concentrated solutions are not recommended as insolubles may form. |
| Other Notes: |
Contains the micro- and macronutrients as well as the vitamins described by Shahin (1984) in his research with tomato protoplasts. He notes the concentration of potassium iodide used in his work as 0.38g/L. This amount is presumed to be a typo due to cross reference of this medium formulation with his patent (#4634674) also describing this medium, but with 0.83 g/L KI. His patent also mentions the addition of casein hydrolysate, which is not included in this formulation. His patent does not include choline chloride at 0.1 mg/L, while it is included in his research paper (1984), and is included in this formulation |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
320 |
| Ammonium Phosphate, Monobasic |
230 |
| Ammonium Sulfate |
134 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
113.25 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA .2H2O |
18.5 |
| Ferrous Sulfate |
13.9 |
| Magnesium Sulfate, Anhydrous |
122.12 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt).2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Zinc Sulfate.7H2O |
8.6 |
| D-Biotin |
0.05 |
| Choline Chloride |
0.1 |
| Folic Acid |
0.5 |
| Glycine (Free Base) |
2.5 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
5 |
| Pyridoxine.HCl |
0.5 |
| Thiamine.HCl |
0.5 |
Application Notes
Plant Tissue Culture Tested This medium was used in Shahin’s (1984) research to regenerate shoots from protoplast derived calli of tomato.
References
Shahin E. A. (1984) Totipotency of tomato protoplasts. Theoretical and Applied Genetics. 69(3): 235-240, DOI: 10.1007/BF00662431. Shahin E. A. (1987) US Patent 4,634,674
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