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TM4 Basal Medium
Product ID: T7954


Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 2.99
Other Notes Plant Tissue Culture Tested
UPC / SKU T7954
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 320
A113 Ammonium Phosphate 230
A305 Ammonium Sulfate 134
B210 Boric Acid
C266 Calcium Chloride, Anhydrous 113.25
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate
E410 EDTA, Disodium Salt 18.5
F263 Ferrous Sulfate, Heptahydrate 13.9
M150 Magnesium Sulfate, Anhydrous
M250 Manganese Sulfate
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
B140 D-Biotin 0.05
C232 Choline Chloride 0.1
F430 Folic Acid 0.5
G503 Glycine 2.5
I703 myo-Inositol 100
N765 Nicotinic Acid 5.0
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.5

T7954 TM4 Basal Medium

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 2.99g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Concentrated solutions are not recommended as insolubles may form.
Other Notes: Contains the micro- and macronutrients as well as the vitamins described by Shahin (1984) in his research with tomato protoplasts. He notes the concentration of potassium iodide used in his work as 0.38g/L. This amount is presumed to be a typo due to cross reference of this medium formulation with his patent (#4634674) also describing this medium, but with 0.83 g/L KI. His patent also mentions the addition of casein hydrolysate, which is not included in this formulation. His patent does not include choline chloride at 0.1 mg/L, while it is included in his research paper (1984), and is included in this formulation
Formula (mg/L)
Ammonium Nitrate  320
Ammonium Phosphate, Monobasic 230
Ammonium Sulfate 134
Boric Acid  6.2
Calcium Chloride, Anhydrous  113.25
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Na2 EDTA .2H2O  18.5
Ferrous Sulfate  13.9
Magnesium Sulfate, Anhydrous  122.12
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Zinc Sulfate.7H2O 8.6
D-Biotin 0.05
Choline Chloride 0.1
Folic Acid 0.5
Glycine (Free Base) 2.5
myo-Inositol  100
Nicotinic Acid (Free Acid) 5
Pyridoxine.HCl  0.5
Thiamine.HCl  0.5

Application Notes
Plant Tissue Culture Tested This medium was used in Shahin’s (1984) research to regenerate shoots from protoplast derived calli of tomato.

Shahin E. A. (1984) Totipotency of tomato protoplasts. Theoretical and Applied Genetics. 69(3): 235-240, DOI: 10.1007/BF00662431. Shahin E. A. (1987) US Patent 4,634,674