TM4 Basal Salts Product ID: T8024
Introduction
Description:
Contains the Macro- and Micronutrients as described by Shahin E. in 1984.
Originally developed for work with Tomato protoplasts.
Glucose can also be added to this formulation, in which is it referred to as TM4G Basal Salt.
This product replaces product T853.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
2.88 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
T8042 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
320 |
| A113 |
Ammonium Phosphate |
230 |
| A305 |
Ammonium Sulfate |
134 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
113.25 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
18.5 |
| F263 |
Ferrous Sulfate, Heptahydrate |
13.9 |
| M150 |
Magnesium Sulfate, Anhydrous |
122.12 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
T8024 TM4 Basal Salt Mixture
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
2.88g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the micro- and macronutrients as described by Shahin (1984) in his research with tomato protoplasts. He notes the concentration of potassium iodide used in his work as 0.38g/L. This amount is presumed to be a typo due to cross reference of this medium formulation with his patent (#4634674) also describing this medium, but with 0.83 g/L KI. Formulation does not include the vitamins, sucrose, agar, MES or plant growth regulators as Shahin’s 1984 formulation describes |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
320 |
| Ammonium Phosphate, Monobasic |
230 |
| Ammonium Sulfate |
134 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
113.25 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA .2H2O |
18.5 |
| Ferrous Sulfate |
13.9 |
| Magnesium Sulfate, Anhydrous |
122.12 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt) .2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Zinc Sulfate.7H2O |
8.6 |
Application Notes
Plant Tissue Culture Tested These micro and macronutrients were used in Shahin’s (1984) TM4 medium while researching the regeneration of shoots from protoplast derived calli of tomato.
References
Shahin E. A. (1984) Totipotency of tomato protoplasts. Theoretical and Applied Genetics. 69(3): 235-240, DOI: 10.1007/BF00662431. Shahin E. A. (1987) US Patent 4,634,674
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