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PhytoTechnology Laboratories

TM4 Basal Salts
Product ID: T8024


Description: Contains the Macro- and Micronutrients as described by Shahin E. in 1984.
Originally developed for work with Tomato protoplasts.
Glucose can also be added to this formulation, in which is it referred to as TM4G Basal Salt.
This product replaces product T853.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 2.88
Other Notes Plant Tissue Culture Tested
UPC / SKU T8042
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 320
A113 Ammonium Phosphate 230
A305 Ammonium Sulfate 134
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 113.25
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 18.5
F263 Ferrous Sulfate, Heptahydrate 13.9
M150 Magnesium Sulfate, Anhydrous 122.12
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6

T8024 TM4 Basal Salt Mixture

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 2.88g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the micro- and macronutrients as described by Shahin (1984) in his research with tomato protoplasts. He notes the concentration of potassium iodide used in his work as 0.38g/L. This amount is presumed to be a typo due to cross reference of this medium formulation with his patent (#4634674) also describing this medium, but with 0.83 g/L KI. Formulation does not include the vitamins, sucrose, agar, MES or plant growth regulators as Shahin’s 1984 formulation describes
Formula (mg/L)
Ammonium Nitrate  320
Ammonium Phosphate, Monobasic  230
Ammonium Sulfate 134
Boric Acid  6.2
Calcium Chloride, Anhydrous 113.25
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA .2H2O  18.5
Ferrous Sulfate  13.9
Magnesium Sulfate, Anhydrous  122.12
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt) .2H2O  0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Zinc Sulfate.7H2O  8.6

Application Notes
Plant Tissue Culture Tested These micro and macronutrients were used in Shahin’s (1984) TM4 medium while researching the regeneration of shoots from protoplast derived calli of tomato.

Shahin E. A. (1984) Totipotency of tomato protoplasts. Theoretical and Applied Genetics. 69(3): 235-240, DOI: 10.1007/BF00662431. Shahin E. A. (1987) US Patent 4,634,674