Sueoka's High Salt Medium (HSM) Product ID: T8224
Introduction
Description: Tris-acetate-phosphate medium (TAP) is a standard maintenance medium often used for Chlamydomonas reinhardtii, the most well-characterized eukaryotic freshwater algae. Ammonium (NH4+) serves as the primary nitrogen source and Tris buffers the pH. Since TAP contains a relatively low concentration of phosphate, it can be used for P labeling as well as experiments/isolations that require clarity of solid-substrate cultures (e.g. agar) (Harris 1989).
See product info sheet for more information about media preparation and other usage.
| Solubility |
Water |
| Physical Form |
Solid |
| Solutions Available |
T8050 (1X) |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
3.17 |
| Other Notes |
Requires 1 mL/L Glacial Acetic Acid (Product # A256) |
| UPC / SKU |
T8224 |
| Synonyms |
Tris-Minimal Medium (if acetate is not added) |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A109 |
Ammonium Chloride |
400 |
| A112 |
Ammonium Molybdate, Tetrahydrate |
1.10 |
| B210 |
Boric Acid |
11.40 |
| C266 |
Calcium Chloride, Anhydrous |
37.74 |
| C350 |
Cobalt Chloride Hexahydrate |
1.610 |
| C375 |
Cupric Sulfate, Pentahydrate |
1.570 |
| E410 |
EDTA, Disodium Salt |
50.00 |
| F263 |
Ferrous Sulfate, Heptahydrate |
4.990 |
| M150 |
Magnesium Sulfate, Anhydrous |
48.83 |
| M455 |
Manganese Chloride, Tetrahydrate |
5.060 |
| P705 |
Potassium Phosphate, Dibasic |
108.0 |
| P846 |
Potassium Phosphate, Monobasic,Anhydrous |
54.0 |
| Z101 |
Zinc Sulfate, Heptahydrate |
22.00 |
| T838 |
Tris Base |
2420 |
|
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