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Life Technologies India
PhytoTechnology Laboratories

Terrestrial (Cypripedium) Orchid Medium,
400 mg/L Calcium Nitrate, without Casein
Product ID: T849


Description: Contains the macro- and micronutrients, glucose, and agar as described by Steele (1996). Contains 400 mg/L Calcium Nitrate. Without Casein.

Solubility Water (Partially)
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 28.44
Other Notes Plant Tissue Culture Tested
UPC / SKU T849
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A114 Ammonium Citrate 19
A300 Ammonium Nitrate 1400
B210 Boric Acid 0.5
C180 Calcium Nitrate 400
C375 Cupric Sulfate, Pentahydrate 0.025
F374 Ferric Ammonium Citrate 25
M150 Magnesium Sulfate, Anhydrous 97.6988
M250 Manganese Sulfate 1.54
P704 Potassium Chloride 100
P840 Potassium Iodide 0.1
P100 Potassium Nitrate 200
P846 Potassium Phosphate, Monobasic, Anhydrous 200
M651 Sodium Molybdate (VI), Dihydrate 0.02
Z101 Zinc Sulfate, Heptahydrate 0.5
A111 Agar 6000
G386 D-Glucose, Anhydrous 20,000

T849 Terrestrial (Cypripedium) Orchid Medium Mother Flasking Medium V

Form: Powder
Appearance: White to Yellow Powder
Application: Orchid Culture
Solubility: Partially Soluble in Wate
Typical Working Concentration: 28.44 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, glucose, and agar as described by Steele (1996).
Contains 400 mg/L Calcium Nitrate
Without Casein
Formula (mg/L)
Ammonium Citrate 19
Ammonium Nitrate 1400
Boric Acid 0.5
Calcium Nitrate 400
Cupric Sulfate.5H2O 0.025
Ferric Ammonium Citrate 25
Magnesium Sulfate, Anhydrous 97.69
Manganese Sulfate.H2O 1.54
MolybdicAcid, Sodium Salt.2H2O 0.02
Potassium Chloride 100
Potassium Iodide 0.1
Potassium Nitrate 200
Potassium Phosphate, Monobasic 200
Zinc Sulfate.7H2O 0.5
Agar 6000
D-Glucose 20,000

Application Notes
Plant Tissue Culture Tested
Plant species: Cypr ipedium and other terrestrial orchid species.

Steele, WK. 1949. In: C. Allen, Editor, North American Native Terrestrial Orchids. Propagation and Production Conf. Proc., pp 11 - 26. (Revised 3/2007)