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PhytoTechnology Laboratories

Tobacco Modified Callus Initiation Medium
Product ID: T856


Description: Contains FeNa-EDTA in place of Ferrous Sulfate and Disodium EDTA.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 5.41
Other Notes Plant Tissue Culture Tested
UPC / SKU T856
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 333
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 181
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
C184 Casein, Enzymatic Hydrolysate 1000
G503 Glycine 2.0
I885 Indole-3-acetic Acid 2.0
I703 myo-Inositol 100
K750 Kinetin 0.2
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.4

T856 Tobacco Modified Callus Initiation Basal Medium

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 5.41g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains FeNa-EDTA in place of Ferrous Sulfate and Disodium EDTA.
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid  6.2
Calcium Chloride, Anhydrous  333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O  0.025
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  181
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt) .2H2O  0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  170
Zinc Sulfate.7H2O  8.6
Casein, Enzymatic Hydrolysate  1000
Glycine (Free Base)  2
Indole-3-acetic Acid  2
Kinetin  0.2
myo-Inositol  100
Nicotinic Acid (Free Acid)  0.5
Pyridoxine.HCl  0.5
Thiamine.HCl  0.4

Application Notes
Plant Tissue Culture Tested Plant species: Nicotianum