3T3-L1

ZenBio now offers Cryopreserved and Cultured 3T3-L1 Preadipocytes!

3T3-L1 murine adipocytes have been fundamental in metabolic disease research for over 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular mechanisms associated with diabetes, obesity and related disorders.

We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using ZenBio's line of 3T3-L1 media.


3T3-Li murine adipocytes
"We have just conducted an experiment with the 3T3L1 cells that we obtained from ZenBio, and we are astounded by the results! In our previous experience with 3T3L1 cells obtained from ATCC and Sigma-Aldrich, it has always been a challenge to get a high percentage of the cells to differentiate into mature adipocytes...we obtained strong differentiation of virtually 100% of the cells to adipocytes on the first try!"
– Vala Sciences, Inc



Ordering Information:

Preadipocytes
Cryopreserved & Cultured 3T3-L1 Preadipocytes
Item Unit Cat#
3T3-L1 Preadipocytes Cryopreserved, 500,000 cells/vial Vial SP-L1-F
3T3-L1 Preadipocytes 96-well plate Plate SP-L1-96
3T3-L1 Preadipocytes 24-well plate Plate SP-L1-24
3T3-L1 Preadipocytes 48-well plate Plate SP-L1-48
  Media
3T3-L1 Adipocyte Medium 500 ml AM-1-L1
3T3-L1 Adipocyte Medium Phenol Red Free 500 ml AM-1-L1-PRF
3T3-L1 Adipocyte Medium WITHOUT Insulin 500 ml AM-1-L1-IF
3T3-L1 Adipocyte Medium WITHOUT Dexamethasone 500 ml AM-1-L1-DF
3T3-L1 Adipocyte Medium WITHOUT Serum 500ml AM-1-L1-SF
3T3-L1 Adipocyte Medium WITHOUT ANTIBIOTICS 500ml AM-1-L1-ABF
3T3-L1 Differentiation Medium 100 ml DM-2-L1
3T3-L1 Differentiation Medium, WITHOUT Phenol Red 500 ml DM-2-L1-500
3T3-L1 Differentiation Medium, WITHOUT ANTIBIOTICS 500ml DM-2-L1-ABF
3T3-L1 Differentiation Medium WITHOUT PPAR gamma agonist 100 ml DM-2-L1-PPG
3T3-L1 Preadipocyte Medium 500 ml PM-1-L1
3T3-L1 Preadipocyte Medium Phenol Red Free 500 ml PM-1-L1-PRF
3T3-L1 Preadipocyte Medium, WITHOUT ANTIBIOTICS 500ml PM-1-L1-ABF
3T3-L1 Preadipocyte Medium, WITHOUT SERUM 500ml PM-1-L1-SF
3T3-L1 Basal Medium 500 ml BM-1-L1
Cryopreservation Medium for 3T3-L1 cells 100 ml FM-1-L1
  Kits
Lipolysis Assay Kit w/ 96 well plate 3T3-L1 cells Kit LIP-1-L1
Lipolysis Assay Kit (Reagents Only) Kit LIP-1-NCL1
3T3-L1 Differentiation KIT (media to differentiate 1 96-well plate) KIT KT-01
3T3-L1 Differentiation KIT (media to differentiate 1 96-well plate) WITHOUT PHENOL RED KIT KT-01-PRF
3T3-L1 Lipolysis Assay KIT (REAGENTS ONLY)-NEFA KIT LIP-2-NC-L1
3T3-L1 Lipolysis Assay KIT -NEFA KIT LIP-2-L1
3T3-L1 Lipolysis Assay KIT (REAGENTS ONLY) Combo-Free Fatty acid and glycerol release KIT LIP-3-NC-L1
3T3-L1 Lipolysis Assay KIT-Combo-Free Fatty acid and glycerol release KIT LIP-3-L1

All ZenBio media are available as without phenol-red (-PRF) and/or without serum (-SF). Please inquire for custom media formulations.

Figure 1 Lipid accumulation in 3T3-L1

Fig. 1. Lipid accumulation in 3T3- L1 cells cultured in ZenBio media.

3T3-L1 preadipocytes were seeded in 24 well plates and induced to differentiate 2 days post confluent using Zen Bio's DM-2-L1 for 3 days. Cells were then fed Zen Bio's AM-1-L1, with fresh media being added every other day. Phase contrast images were taken on day 7 (Panel A) and day 14 (Panel B) of differentiation using an Olympus IX60 microscope equipped with a STOP digital camera (20X magnification)