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BrilliantDye™
Terminator Cycle Sequencing Kit
Improved performance
compared to all existing competitors |
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Single Replacement for all
existing Dyes!!
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Robust and early start
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Combined with long reads
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No need for changes in protocol, settings or calibration
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Based on mobilities of existing versions
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Ultra-competitive pricing
Introduction
The BrilliantDyeTM
Terminator Cycle Sequencing Kit is a complete kit, based on the trusted
Sanger
Chain Termination method.
Format
The kit is delivered as a 2.5x concentrated
ready-re柑ction premix, fully optimized for a highly flexible
chemistry, designed for all kinds of Sequencing applications, including
de novo sequencing and resequencing. The kit generates data with
uniform peak heights and optimized signal balance to pro查uce long,
high-quality reads.
Increased
performance
By combining the robustness and better mobility
of the dye set, with a novel enzyme and optimized dNTP/ddNTP ratios for
longer reads, BrilliantDye is the universal Sequencing kit that can be
used for all kinds of applications:
- Sequencing
of (short) PCR products with fast, rapid, or ultra-rapid run modules
and High Quality Base Calling for the small fragments.
- Sequencing of (long range) PCR products with
maximum read lengths with standard or long run modules.
- Sequencing of Plasmid DNA with maximum read
length.
- Sensitive Heterozygote detection with optimized
peak heights distribution.
Kit content
The BrilliantDye Terminator Cycle Sequenc枰ng
Kit contain all required reagent components for the sequencing reaction
in a ready reaction, pre-mixed format:
- Reaction Ready seq. premix
- 5X sequencing buffer
- pGem control
- M13(-21) primer
Dye set
The kit is optimized to run with various Filtersets (Refer to protocol).
Ordering Information
Cat. no.
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Reactions
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RR seq. Premix
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5 x Seq. Buffer
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pGem control
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M13(-21) primer
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BRD1-024
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24
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1 x 192 無
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1 x 0.65 mL
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1 x 10 無
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1 x 10 無
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BRD1-100
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100
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1 x 800 無
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1 x 2.0 mL
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1 x 10 無
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1 x 10 無
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BRD-1000
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1000
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10 x 800 無
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8 x 2.0 mL
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1 x 50 無
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1 x 50 無
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BRD1-5000
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5000
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50 x 800 無
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40 x 2.0 mL
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5 x 50 無
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5 x 50 無
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Instrument platforms: The purified extension products can be analyzed by capillary electrophoresis on any of the following platforms:
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Applied Biosystems 310 DNA sequencer
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Applied Biosystems / Hitachi 3100 (Avant) Genetic Analyzer
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Applied Biosystems / Hitachi 3130 (XL)
Genetic Analyzer
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Applied Biosystems / Hitachi 3500 (xL) Genetic Analyzer
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Applied Biosystems / Hitachi 3730 (XL) DNA Analyzer
*Applied Biosystems
is a product of Thermo Fisher Scientific Inc.
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