Hy-DNase I |  | Cat
No. N9066
Size:
1 gm Specific
Activity: ≥
2000 Kunitz units/mg Form: Contains
1g Hy-DNase I, Lyophilized Powder
Store
at 4°C
Description Hy-DNase
I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di, tri- and oligonucleotide
products with 5´-phosphorylated and 3´-hydroxylated ends. Hy-DNase I acts on
single- and double-stranded DNA,
chromatin and RNA: DNA hybrids.
Features
- Recombinant enzyme.
- Purified from non-animal host with a lower level of intrinsic RNases.
Applications
- Degradation of DNA template in transcription reactions
- Removal of contaminating genomic DNA from RNA samples
- DNase I footprinting
- Nick Translation
Definition
of Activity Unit One
unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37°C.
Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0),
10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA.
Source An E.
Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.
Molecular
Weight 29
K Da monomer.
Quality
Control The
absence of ribonucleases is confirmed by appropriate quality test, functionally
tested for digestion of template DNA after in vitro transcription.
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