Hy-DNase I
Cat No. N9066

Size: 1 gm
Specific Activity:  ≥ 2000 Kunitz units/mg
Form: Contains 1g Hy-DNase I, Lyophilized Powder

Store at 4°C

Description
Hy-DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. Hy-DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.

Features
  • Recombinant enzyme.
  • Purified from non-animal host with a lower level of intrinsic RNases.
Applications
  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation
Definition of Activity Unit
One unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA.

Source
An E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.

Molecular Weight
29 K Da monomer.

Quality Control
The absence of ribonucleases is confirmed by appropriate quality test, functionally tested for digestion of template DNA after in vitro transcription.