Hy-Fy
High Fidelity Mix (x2) |
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#EZ-2021
Contents: | 2X High Fidelity Mix | 1ml | | Nuclease-free water | 1ml |
Store at -20°C Shelf life: 2 years
Description
Hy-Fy
High Fidelity Mix (x2) is a premixed, ready-to-use solution containing High
Fidelity DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal
concentrations for efficient amplification of DNA templates by PCR. To prepare
the final PCR, only primers and template DNA are added. High Fidelity Mix
contributes to highly reproducible PCR by reducing the risk of pipetting
errors, miscalculation and contamination. It also contributes to higher
sensitivity by adding intensifier and optimizer. High Fidelity DNA polymerase,
derived from the hyperthermophilic archae Pyrococcus furiosus, has been
shown to exhibit superior thermostability and proofreading properties compared
to other thermostable polymerase. Unlike Taq DNA polymerase, highly
thermostable High Fidelity DNA polymerase possesses 3' to 5' exonuclease
proofreading activity that enables the polymerase to correct
nucleotide-misincorporation errors. This means that High Fidelity DNA
polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR
inserts. Use of High Fidelity DNA polymerase in your PCR reactions results in
blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors.
High Fidelity DNA polymerase is superior for techniques that require
high-fidelity DNA synthesis.
Features
- Convenient –High Fidelity DNA Polymerase in a ready-to-use Mix.
- High yields of PCR products with minimal optimization.
- Fast -saves time due to reduced number of pipetting steps.
- Reproducible -lower contamination and pipetting error risk
Applications
- High fidelity PCR
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
- Site-directed mutagenesis
Composition of the 2x High Fidelity Ready Mix High
Fidelity DNA polymerase is supplied in 2X High Fidelity buffer, dNTPs,
3 mM MgSO4 and bromophenol blue. High Fidelity mix buffer is a
proprietary formulation optimized for robust performance in PCR.
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