Hy-Fy High Fidelity Mix (x2)

Contents:2X High Fidelity Mix      1ml
Nuclease-free water1ml

Store at -20C
Shelf life: 2 years

Hy-Fy High Fidelity Mix (x2) is a premixed, ready-to-use solution containing High Fidelity DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. High Fidelity Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer. High Fidelity DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable High Fidelity DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that High Fidelity DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Use of High Fidelity DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. High Fidelity DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

  • Convenient –High Fidelity DNA Polymerase in a ready-to-use Mix.
  • High yields of PCR products with minimal optimization.
  • Fast -saves time due to reduced number of pipetting steps.
  • Reproducible -lower contamination and pipetting error risk

  • High fidelity PCR
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning
  • Site-directed mutagenesis
Composition of the 2x High Fidelity Ready Mix
High Fidelity DNA polymerase is supplied in 2X High Fidelity buffer, dNTPs, 3 mM MgSO4 and bromophenol blue. High Fidelity mix buffer is a proprietary formulation optimized for robust performance in PCR.