Gel/PCR Extraction Kit |
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EX-GP200 (200rxns)
Content
Content | EX-GP200 | Binding
Buffer BD | 80
ml | Wash
Buffer PE | 20
mlx3 | Elution
Buffer (10 mM Tris-HCl, pH 8.5) | 10
ml | Spin
Columns | 200
each |
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Description
Gel
/PCR Extraction Kit is designed to extract and purify DNA from standard
or low-melt agarose gels in either Tris acetate (TAE) or Tris borate
(TBE) and from PCR and other enzymatic reaction mixtures. This
membrane-based system, which can bind up to 40μg DNA, allows recovery
of isolated DNA in as little as 20 minutes, depending on the number of
samples processed and the protocol used. The purified DNA can be used
for automated fluorescent DNA sequencing, cloning, labeling,
restriction enzyme digestion or in vitro transcription/translation
without further manipulation.
Features
- Fast - procedure takes only 15 min.
- High Efficient – up to 85% recoveries in the range of 50bp-40kb.
- High purity - OD260/280=1.7-1.9. Purified DNA is ready for downstream application such as PCR, restriction digestion
Applications Fast and efficient extraction of high purity DNA ideal for use in all conventional molecular biology procedures including:
- Conventional restriction digestion
- Automated fluorescent or radioactive sequencing
- PCR
- DNA Sequencing
- In vitro transcription
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