Hy-RNase
A |
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Cat
No. N9046
Size:
100mg
Contents: Hy-RNase A: 100mg
Specific Activity: >2500 u/mg protein (>50 Kunitz
units/mg protein)
Form: Contains 100 mg Hy-RNase A, Lyophilized Powder
Description
Hy-RNase
A, DNase and protease-free is an endoribonuclease that specifically
degrades
single-stranded RNA at C and U residues. It cleaves the phosphodiester
bond
between the 5'-ribose of a nucleotide and the phosphate group attached
to the
3'-ribose of an adjacent pyrimidine nucleotide, resulting 2', 3'-cyclic
phosphate hydrolyzed to the corresponding 3'-nucleoside phosphate.
Features
Hy-RNase
A is free of DNase activity. It is not necessary to heat it before use.
Applications
-Plasmid
and genomic DNA preparation
-Removal
of RNA from recombinant protein preparations.
-Ribonuclease
protection assays
-Mapping
single-base mutations in DNA or RNA
Definition
of Activity Unit
One
unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm
when yeast Hy-RNA is hydrolyzed at 37°C and pH 5.0.
Fifty units are approximately equivalent to 1 Kunitz unit.
Storage
Stable
at room temperature for one year. For long time storage, store the
Hy-RNase A powder at 4°C.
Source
Bovine
pancreas.
Molecular
Weight
13.7
kDa monomer.
Quality
Control
The
absence of endodeoxyribonucleases, exodeoxyribonucleases and proteases
confirmed by appropriate quality tests, functionally tested for RNA
digestion in a plasmid DNA purification procedure.
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