SuperiorScript II Reverse Transcriptase

SuperiorScript II Reverse Transcriptase is genetically engineered version of M-MLV RT which is reduced RNase H activity and increased thermostability, thus this enzyme can synthesize cDNA at high temperatures more than M-MLV RT. SuperiorScript II Reverse Transcriptase shows improved cDNA yields and RNA detection sensitivity. This enzyme is capable of synthesizing cDNA longer than 12.3 kb from messenger RNA.

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Thermostability of SuperiorScript II on 3 targets. cDNA was synthesized from 500 ng total HeLa RNA for beta-actin 184bp, GAPDH 395 bp and TFR 738 bp. One-tenth of the cDNA reaction was used for 30 cycles of PCR with nTaq-multi HOT	Sensitivity of SuperiorScript II. cDNA was Synthesized from HeLa total RNA with oligo(dT) primer using buffer supplied with each RT and recommended conditions.10% of cDNA reaction was added to 20ul PCR reaction containing primers for 500bp GAPDH gene and 2X TOPsimple™ DyeMIX-HOT.