SuperiorScript III Reverse Transcriptase

SuperiorScript III Reverse Transcriptase is genetically engineered version of M-MLV RT which is reduced RNase H activity and increased thermostability, thus this enzyme can synthesize first-strand cDNA at elevated temperatures up to 55 ?. SuperiorScript III Reverse Transcriptase shows improved cDNA yields and RNA detection sensitivity than the competitor’s enzyme. This enzyme is capable of synthesizing cDNA from 100 bp to 12 kb or more.

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Thermostability of SuperiorScript III on 3 targets. cDNA was synthesized from 500 ng total HeLa RNA for beta-actin 184 bp, GAPDH 395 bp and TFR 738 bp. One-tenth of the cDNA reaction was used for 30 cycles of PCR with nTaq-multi HOT.	Sensitivity of SuperiorScript III. cDNA was Synthesized from HeLa total RNA with oligo(dT) primer using buffer supplied with each RT and recommended conditions. 10% of cDNA reaction was added to 20 µl PCR reaction containing primers for 500 bp GAPDH gene and 2X TOPsimple™ DyeMIX-HOT.