TOPscript™ cDNA Synthesis Kit cDNA Synthesis Kit is designed for the highest efficiency conversion of RNA to cDNA. TOPscript™ Reverse Transcriptase is genetically engineered version of M-MLV Reverse Transcriptase which is highly thermostable, thus can synthesize cDNA at elevated temperatures up to 60°C A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source, for maintaining archival cDNA, and for providing maximum flexibility in selecting a downstream qPCR reagent system. The TOPscript™ cDNA synthesis Kit is formatted for high-yield production of cDNA up to 20 kb and each reaction accommodates a range of RNA amounts from fg to µg.
|
