BiOstic® Stabilized Blood RNA Isolation Kit

Overview

For isolation of total RNA from blood, buffy coat cells, bone marrow, and PAXgeneT Blood RNA Tube* collected samples.
 

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Features
  • Greater RNA yield compared to other PAXgene™ blood RNA tube extraction methods
  • Enhanced mechanical lysis using our novel silica carbide DNA shearing column breaks down viscosity and releases more RNA for binding
  • Proprietary cell pellet wash buffer ensures recovery and integrity of cells before extraction
  • Available in 20, 50 or 100 prep sizes
  • Complete and readyto-use kit includes DNase I and prepared wash buffer for more consistent results

Description



The BiOstic® Stabilized Blood RNA Isolation Kit is designed for high yield isolation of total RNA from whole blood collected in PAXgene™ Blood RNA Tubes*. A ready-to-use and specially formulated was buffer ensures reliable and complete collection of cells at the start of the protocol and a novel silica carbide DNA shearing column provides more complete RNA solubilization and release for increased binding. Due to these improvements, the BiOstic® Stabilized Blood RNA Isolation Kit will purify total RNA from 2.5ml of stabilized blood with higher yields as compared to competitor kits (e.g. Competitor Q PAXgene™ Blood RNA kit).The BiOstic® Stabilized Blood RNA Isolation Kit is sold for research use only. *Kit does not include PAXgene™ tubes. PAXgene™ is a registered trademark of Qiagen

Specifications


Format Silica Spin Filter Tubes
Method Enzymatic and bead homogenization
Sample Size One PAXgene™ Blood RNA Tube
Binding Capacity Up to 20 μg per spin filter
Time 65 minutes
Equipment Required Vortex, centrifuge, and water bath or heat block

Storage Conditions


Remove lyophilized DNase I and store at 4ºC. Proteinase K is stable at room temperature. For prolonged storage, place the Proteinase K at 4ºC. Store all other reagents and kit components at room temperature (15-30°C). Note: After resuspension, store DNase I at -20°C (DNase I is sensitive to physical denaturation. Do not vortex the resuspended DNase I).

Kit Components
Component 12230-50
Solution PX1 220 ml
Solution PX2 20 ml
Solution PX3 20 ml
Solution PX4 20 ml
Solution PX5 46 ml
Solution PX6 3 ml
Solution PX7 2 x 28 ml
Solution PX8 6 ml
DNase I (RNase-Free) 1500 units
Proteinase K Solution 2.25 ml
Carbide Bead Tubes 50
Spin Filters 50
2 ml Collection Tubes 150
DNA Shearing Columns 50