Overview
Isolate RNA from up to 2 ml of whole blood or 10 million cells
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Features
- Purify RNA from up to 2 ml of whole blood or 10 millions cells
- Fast and easy format with novel MO BIO flat bottom spin columns
- DNase 1 (Rnase-Free) included
- Isolate RNA from whole blood in 45 minutes
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Description
The BiOstic® Blood Total RNA Isolation Kit provides a way to purify RNA from up to 2 ml of whole blood or 10 millions cells using a fast and easy spin column.
This method uses a simple hypotonic red blood cell (RBC) lysis to obtain a white blood cell (WBC) pellet for extraction of RNA.
Silica Spin column products utilize the novel MO BIO flat bottom spin column design, which provides improved samples processing and yields. The bucket configuration allows for enhanced samples flow and membrane drying after wash step since the entire membrane is accessible for air flows.
The silica technology provides a robust and fast way to purify nucleic acids without the use of organic solvents or cesium chloride gradients.
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Research and Development |
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Figure 1: RNA yields were twice as high compared to Qiagen using the BiOstic® Blood Total RNA Isolation Kit. Lane
1: Ladder; lane 2: MO BIO, avg. yield of 2.5 μg; lane 3: Qiagen, avg. yield 1.2 μg; lane 4: Invitrogen, avg. yield 1.6 μg |
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Figure 2: Successful RT-PCR with the BiOstic® Blood Total RNA Isolation Kit. RNA isolated is ready to use for enzymatic applications. |
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Specifications |
Competition Spec |
MO BIO |
SupplierQ |
Supplier I |
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Starting volume of blood: up to |
2 ml |
1.5 ml |
500 μl |
Protocol speed |
45 minutes |
60 minutes |
53 minutes |
Dnase included? |
YES |
NO |
NO |
Ready to use? |
YES |
NO |
NO |
Average Yield (Fig. 1) |
2.5 μg |
1.2 μg |
1.6 μg |
Purity 260/280 |
2.02 |
1.92 |
1.62 |
Purity 260/230 |
1.61 |
1.14 |
0.65 |
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Storage Conditions |
Store RNase at 4°C. Store all other components at room temperature.
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Kit Components |
Component |
12230-50 |
12230-20 |
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Solution BR1 |
1 x 550 ml |
1 x 220 ml |
Solution BR2 |
1 x 40 ml |
1 x 16 ml |
Solution BR3 |
2 x 20 ml |
1 x 16 ml |
Solution BR4 |
2 x 23 ml |
1 x 18 ml |
Solution BR5 |
1 x 2.5 ml |
1 x 1 ml |
Solution BR6 |
2 x 28 ml |
1 x 22 m1 |
Solution BR7 |
1 x 8 ml |
1 x 3 ml |
DNase 1 |
1500 units |
750 units |
Spin Filters |
50 |
20 |
2 ml Collection Tubes |
150 |
60 |
15 ml Collection Tubes |
50 |
20 |
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