Break Through in RNA Isolation One-Step Method without Phase Separation

Product Description

RNAzolŪRT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patent-pending reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzolŪRT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment. RNAzolŪRT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. Supplementing RNAzolŪRT with water makes DNA, proteins and polysaccharides insoluble.
A biological sample is homogenized or lysed in RNAzol®RT. DNA, proteins, polysaccharides and other molecules are precipitated from the homogenate/lysate by the addition of water and removed by centrifugation. The pure RNA is isolated from the resulting supernatant by ethanol precipitation, followed by washing and solubilization.

  • The isolation procedure can be completed in less than one hour. The isolated RNA is ready for use in RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
  • RNAzol®RT isolates total RNA, with mRNA and small RNA as separate fractions. Small RNA contains RNA <200 bases, including 10 - 15 base miRNA.
  • In addition, RNAzol®RT allows for the simultaneous isolation of RNA and DNA.
  • The RNAzol®RT procedure is performed at room temperature, including centrifugation.
  • Due to lack of co-isolation of impurities, the RNAzol®RT procedure yields RNA pellets that are significantly
STABILITY/STORAGE: RNAzol®RT is stable when stored at room temperature for at least two years from date of purchase.