MEM, DMEM, RPMI-1640, IMDM, McCoy's, Medium M-99, F-10, Ham's & DPBS

Biological Industries' medium products are prepared by a sterile filtration process and aseptic filling. The process has been validated to ensure that the production of solutions meets the sterility assurance level of 103. The filtration step includes the use of sterilizing grade membrane filters. After filtration, the medium is dispensed into bottles by an aseptic fillingprocess which has been validated to insure sterility of the final product. Medium products are produced in a controlled environment (clean rooms) designed to carefully control air pressure and particulate matter. The manufacturing area is a class 100,000 (ISO 8) environment. The sterile bottles and equipment are stored in a class 10,000 (ISO 7) environment, and the filling room is a class 1000 (ISO 6) environment with class 100 (ISO 5) laminar air flow sterile bench. Clean rooms are monitored on a regular basis for particulate and microbial levels to ensure that the air handling system, cleaning protocols and personnel maintain required standards. After filling, the final product is held in quarantine until all quality control tests have been completed.

Physicals Tests

Endotoxins

Sterility Testing

Cell Growth Promotion

Stability

Expiration Date -

Storage :

Certificate of Analysis and Safety Data

Media Preparation

• Measure out sterile culture - grade water (Catalogue No. 03-055-1) to approximately 70% of desired total volume of media. Pour water into an appropriate sterile mixing container that is close to the desired final volume. The water should be at room temperature.
• Add the amount of the concentrated medium or concentrated medium components.
• Add the desired amount of L-Glutamine Solution 200 mM (Catalogue No. 03-020-1) if required.
• Add the desired amount of Sodium Bicarbonate Solution 7.5% (Catalogue No. 03-040-1).
• Add antibiotics solution if desired.
• Add water to the final volume. During the dilution, stir gently into equilibrium. If necessary, adjust pH with sterile 1 N NaOH or HCI.
• Add the desired amount of serum, if required.
• Store at 2°C to 8°C.
  
  Important The above procedures are carried out under strict sterile conditions. Do not use mouth pipetting.

Example 1

Preparation of Basal Medium-Eagle, Earle's Salt Base, one liter

• 700 ml sterile water (Catalogue No.03-055-1)
• . 100 ml Basal Medium-Eagle, Earle's Salts Base, concentrate 10X, without Sodium Bicarbonate and L-Glutamine (Catalogue No. 01-015-5).
• 10 ml L-Glutamine Solution 200 mM (Catalogue No. 03-020-1).
• 29.4 ml Sodium Bicarbonate Solution 7.5%. (Catalogue No. 03-040-1).
• . 10ml Penicillin-Streptomycin Solution (Catalogue No. 03-031-1).
• . Sterile water to final volume, Adjust pH if necessary.

Example 2

• 700 ml distilled water
• Add 100 ml RPMI Concentrate 10X
• Adjust pH to 6.5-7.0 with 1N NaOH
• Add 10.3 ml L-Glutamine Solution 200 mM
• Add 26.7 ml Sodium Bicarbonate Solution 7.5%
• Adjust pH with 1N NaOH or 1N HCI to pH 7.0-7.4
• Add distilled water to final volume. Adjust pH if necessary.
• Filter the medium into sterile containers using a 0.2 μm membrane filter.

• Can be used as a direct substitute for L-Glutamine at equimolar concentrations in mammalian cell culture systems.
• Eliminates problems associated with the spontaneous breakdown of L-Glutamine during incubation.
• Highly soluble in aqueous solution and is heat stable.

Expiration

Storage

EPBS, HBSS, DPBS, GBSS AND SEVERAL OTHERS

Balanced Salt Solutions, for all intents and purposes, are inorganic salt solutions that form the basis of many complex media formulations. They may
contain varying amounts of NaCl, KCl, MgCl2, NaHCO3, MgSO4, CaCl2 and other salts, and have since been modified and enriched along with amino
acids, vitamins, fatty acids and lipids and as well as other nutrients that segue into a final medium based upon application and technique to meet
the cells unique niche requirements. These precise media formulations are now optimized to the nth degree to support a wide array of cell lines. The
current role of a balanced salt solution in cell culture is multi-faceted and may be divided into four principal functions.

• Functions as a diluent, as an irrigating medium or transporting fluid while maintaining osmoregulation, the optimal and constant balance of osmotic pressure gradients between the intracellular and extracellular compartments.
.
• Provides cells with fluids and certain bulk inorganic ions essential for normal cell metabolism.
• When combined with a carbohydrate, such as Glucose (C6H12O6), it provides a primary energy source for cell metabolism.
• When provided with a buffering system, it facilitates the maintenance of physiological pH within an acceptable range of 7.1-7.5.