Mesenchymal Stem Cell (hMSC) Products

Certified Fetal Bovine Serum (FBS) Qualified for hMSCs

The USDA grade certified Fetal Bovine Sera (FBS) qualified for MSC are high quality prescreened FBS batches. It saves time and efforts by eliminating the need to test multiple lots for performance.

MSC qualified FBS are screened for the following:

  • Ability to differentiate
  • Morphology
  • Cell growth and expansion
  • Clonal efficiency
Each lot of FBS is extensively tested for its ability to support in vitro growth and expansion of undifferentiated hMSCs in two culture systems:
I. Promotion of cell growth and expansion
Figure 1. hMSC-BM cultured in DMEM low-Glucose supplemented with 10% of Biological Industries’ MSC Qualified FBS
II. Clonal efficiency
MSC Colony Forming Unit-Fibroblast (CFU-F) assay was used to evaluate the self renewal potential of hMSC in tested conditions. Optimized serum will induce higher numbers of MSC colonies when cultured at very low seeding density.
Figure 2. Represented colony stained with 0.5% crystal violet following 14-day CFU-F assay of hMSC-AT cultured in DMEM low-glucose supplemented with 10% of Biological Industries’ MSC Qualified FBS.

MSC Freezing Solution

A chemically defined, animal component-free and proteinfree formulation for optimal cryopreservation of hMSC

  • A complete, ready-to-use solution
  • Protein-free
  • Non-animal or human derived components
  • Suitable for cells cultured in both serum-free and serumcontaining media
  • High cell viability after thawing
Figure 3. hMSC-AT frozen in MSC Freezing Solution; 2 days after thawing (in serum-free medium)

Xeno-Free MSC Attachment Solution

A xeno-free solution for attachment and spreading of hMSC in serum-free conditions

  • Ready-to-use solution
  • Xeno-free
  • Designed for use in serum-free culture systems
Figure 4. hMSC-BM 8 days from seeding on plates coated with MSC Attachment Solution growing on serum-free medium
Trypsin Substitutes for Cell Dissociation

MSC Dissociation Solution (Plant Enzyme Derived)

Optimized Animal componentfree (ACF) formulation for efficient detachment of hMSCs from culture vessels.
  • Ready-to-use solution
  • No animal or human derived components
  • Contains plant-derived enzyme
  • Works with both serum-free and serum-containing culture systems
  • Allows high viability and rapid detachment of single cell suspension

MSC Dissociation Solution (Non-Enzymatic)

A chemically defined, animal component-free formulation for gentle and efficient dissociation of hMSCs

  • Ready-to-use solution
  • Chemically defined
  • Contains a proprietary mixture of chelators
  • Suitable for both serum-free and serum-containing culture systems
  • Reduces the risk of cell damage associated with enzyme based dissociation solutions