PowerPlant® DNA Isolation Kit

Overview

For isolating quality DNA from plants including those high in phenolics and polysaccharides.
 

Features
  • New method improves PCR inhibitor removal
  • Included steel beads enhance sample homogenization
  • Utilizes easy hands-off homogenization
  • Homogenization method eliminates crosscontamination and the need to clean grinding tools between samples
  • Includes tough customized tubes to bead beat and homogenize without tube breakage
  • Process more samples with less clean up
  • Compatible with vortexes, MO BIO’s Precellys® 24 or any bench top homogenizer

Description
The PowerPlant® DNA Isolation Kit isolates genomic DNA free from PCR impurities from even the toughest plant samples using a powerful homogenization method. Due to the unique hands-off bead beating method, up to 24 samples can be processed simultaneously using a vortex adapter or a bench top homogenizer, such as our Precellys® 24 Homogenizer. This method effectively eliminates both bottlenecks and crosscontamination risk found with hand grinding with a mortar and pestle.

The PowerPlant® DNA Isolation Kit saves significant time when compared to traditional liquid nitrogen methods and yields are comparable. In addition, its novel PCR inhibitor removal system ensures the DNA is free of polyphenols and polysaccharides allowing for all downstream applications including successful PCR analysis.

Other Products
  • UltraClean-htp™ 96 Well Plant DNA Isolation Kit
  • UltraClean™ Plant DNA Isolation Kit
  • PowerPlant™ DNA Isolation Kit Components
  • UltraClean™ Plant DNA Kit Components
  • PowerMax™ Soil DNA Isolation Kit

Powerful Protocol
The PowerPlant® DNA Isolation Kit protocol begins by adding plant material to the provided tubes containing specialized beads along with optimized reagents for rapid homogenization. Cell lysis and DNA release occur by both mechanical and chemical methods to maximize DNA isolation efficiency. A silica membrane in a spin column format is used to capture, wash and elute the pure DNA.

The PowerPlant® DNA Isolation Kit has been successfully utilized on a wide variety of plant types including leaf, roots, and seed samples. Occasionally, plants such as pine needles, cotton, sunflower, and strawberry present additional challenges and require an additional clean up procedure. The PowerPlant® DNA Isolation Kit provides special clean-up reagents as an optional step to assure success among even the most challenging plant samples.

Specifications
Format Silica Spin Filter Tubes
Method Bead Beating
Starting Amount 50 mg
Binding Capacity Up to 40 μg per filter
Throughput 1 - 24 samples
Time 60 minutes
Equipment
Required		 Vortex and Vortex
Adapter
 

Storage Conditions
Store at room temperature for up to 2 years.

Kit Components
Component 13200- 50 13200- 100
Bead Tubes 50 100
Bead Solution 30 ml 70 ml
Solution PB1 5.5 ml 11 ml
Solution PB2 20 ml 20 ml
Solution PB3 3 x 30 ml 6 x 30 ml
Solution PB4 28 ml 55 ml
Solution PB5 30 ml 2 x 30 ml
Solution PB6 11 ml 22 ml
Spin filters in Tubes 4 ml 8 ml
2 ml Collection 50 100
Tubes 100 200
2.2 ml Collection Tubes 50 100
 

Publications
  • Samuels, G.J., Lu, B-S., Chaverri, P., Candoussau, F., Fournier, J., and Rossman, A.Y. Cyanonextria ad New Genus for Nectria cyanostoma and its Fusarium Anamorph. Mycological Progress. 8(1): 49-58 (2009).
  • Desalvo, M.K., Voolstra, C.R., Sunagawa, S., Schwarz, J.A., Stillman, J.H., Coffroth, M.A., Szmant, A.M., and Medina, M. Differential gene expression during thermal stress and bleaching in the Caribbean coral Montastraea faveolata. Molecular Ecology. 17: 3952-3971 (2008).