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Concentrate dilute, aqueous RNA samples to small volumes
- Concentrate small amounts of RNA (³10 pg in 500 μl) to only 10 μ
- Minimize sample loss – no wash steps
- Protect RNA integrity during concentrating process
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Key Benefits: |
- Minimize loss of starting material
- Protect RNA integrity during the concentrating process
- Store dried samples at room temperature for up to 1 week
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Product Features: |
- Format: Individual 1.7 ml snap-cap tubes
- Sample source: Purified total or poly(A) RNA aqueous solutions
- Initial sample size: Up to 50 μg RNA in a maximum starting volume of 500 μl
- Rehydration volume: Minimum of 10 μl
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RNAconcentrator is designed to concentrate dilute, aqueous RNA solutions from picogram amounts of starting material to only 10 μl. The unique stabilization medium allows for improved recovery as compared to traditional methods, as the thermo-stable properties of RNAconcentrator protect samples from further degradation. It is easy to use - samples are applied into the RNAconcentrator tube and dried down. The dried RNA sample can then be conveniently stored for up to 1 week at room temperature until ready for use. Concentrated RNA can be used directly in downstream applications without further purification, thus avoiding sample loss typically associated multiple wash steps.
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Concentration of Picogram Amounts of RNA
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| Comparison of Concentration Methods. (Left) Aliquots of 10 pg of purified total RNA in 100 μl volume was concentrated using RNAconcentrator or into an empty tubes (SpeedVac), and also using ethanol precipitation (EtOH ppt). Concentrated samples were rehydrated in 10 μl of water and 2.5 μl of each sample was used for quantitative RT-PCR analysis using TaqMan reagents (18S ribosomal RNA). Results indicate significantly improved recovery of concentrated RNA using RNAconcentrator as compared to conventional methods. (Right) Aliquots of ~100 ng of purified total RNA in a 100 μl volume was concentrated using a SpeedVac into tubes with RNAconcentrator or into an empty tubes (SpeedVac), and also using ethanol precipitation (EtOH ppt). Concentrated samples were resuspended in 10 μl water and used as input template for reverse-transcription PCR (RT-PCR) for amplification of the human Hsp90 amplicon (2.3 kb). Results indicate more abundant amplification from RNA templates concentrated with RNAconcentrator as compared to conventional methods. |
Procedure: |
- Dilute, aqueous solutions of purified total or poly(A) RNA are applied into tubes containing RNAconcentrator from as little as 10 pg starting material in a maximum volume of 500 μl.
- Samples are then dried using a vacuum concentrator and can then be conveniently stored for up to 1 week at room temperature.
- Concentrated RNA can be rehydrated in only 10 μl in appropriate buffer or water and used immediately without further purification.
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Applications: |
| RNAconcentrator is compatible with the following applications: |
- Quantitative RT-PCR (e.g. TaqMan®, SYBRGreen®)
- Quantitation analysis (e.g. Qubit™ platform, Ribogreen®)
- Bioanalyzer and microarray analysis
- Reverse transcription • cDNA synthesis
- Agarose gel electrophoresis
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RNAconcentrator is available in the following format: |
| 93421-003: RNA Concentrator Tube Kit (25 tubes) |
