General Purpose Western Blot Reagents |
General Purpose Western Blot Reagents |
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Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA
Western Accessories |
Catalog# |
Product Name. |
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80081 |
Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X) |
100 ml |
80082 |
Tris Buffered Saline (TBS, pH 7.4) concentrate (10X) |
1 L |
80215 |
NuGlo ECL Substrate Kit for Western blot, 400 mls |
1 kit |
80215-250 |
NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls |
1 kit |
80400-100 |
Bovine Serum Albumin (BSA, protease-free) for western (makes ~2-L buffer @5% BSA) |
100 g |
80401-250 |
Blotto for Western/ELISA (non-fat milk, makes ~25-L buffer @5%) |
250 g |
80402-500 |
Blotto 1 buffer (5% milk, 0.05% tween in TBS buffer) for Western |
500 ml |
80403-500 |
Blotto 2 buffer (1% milk, 1% BSA, 0.05% Tween in TBS for phosphoproteins) for WB |
500 ml |
ALP15-N-1 |
Alkaline phosphatase (AP), calf intestine, Conjugation grade (3000-6000 u/mg) |
1 mg |
ALP16-N-1 |
Alkaline phosphatase (AP), calf intestine, Mol Biol grade (3000-6000 u/mg) (Free of endonuclease, exonuclease and RNase activities) |
1 mg |
GO11-N-250 |
Glucose Oxidase (GO) enzyme (~200-300 u/mg) |
250 mg |
LMWM-12 |
Low range multi-color protein markers (6.5-45 kDa) for Western |
250 ul |
HMWM-11 |
High range multi-color protein markers (6.5-205 kDa) for Western |
250 ul |
HRP15-N |
Horse Radish Peroxidase (HRP) Enzyme, purified |
1 mg |
HRP16-N-500 |
Horse Radish Peroxidase (HRP) Enzyme (~200 u/mg) |
500 mg |
HRPS-100 |
Antibody/Protein-Peroxidase-Conjugate stabilizer solution |
100 ml |
PRTS-100 |
Coating Antibody or Protein stabilizer solution (10X) |
100 ml |
RIPA-50 |
RIPA lysis buffer kit for mammalian cell lysis (complete with protease inhibitor cocktail (PMSF, Protease inhibitors, Orthovanadate) |
50 ml |
SALL-500 |
StainAll dye for the visualization of proteins on membranes (transfer) |
500 ml |
TW-100110 |
Tween-20 (50%, convenient to dispense) for ELISA, Western, IP etc |
500 ml |
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Western Blot Kits for Human & Animals |
Catalog# |
Product Name. |
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80200 |
Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate |
1 kit |
80201 |
Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate |
1 kit |
80202 |
Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate |
1 kit |
80203 |
Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate |
1 kit |
80204 |
Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate |
1 kit |
80205 |
Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate |
1 kit |
80206 |
Western blot Kit for G. pig Primary Antibodies, Chemilum. Substrate |
1 kit |
80207 |
Western blot Kit for Human Primary Antibodies, Chemilum. Substrate |
1 kit |
80208 |
Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate |
1 kit |
80209 |
Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate |
1 kit |
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Notes: Items # 80202-80209 provide all necessary reagents (blocking buffer, primary antibody diluent, wash buffer concentrate, species specific secondary antibody-HRP Conjugate, and NuGlo ECL Substrate) to perform Western Blots. It has sufficient reagents to process 2000-4000 cm2 (10-20 mini blots) or 200-400 single lane strips.
Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under Western Blot Recycling kit |
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General Purpose ELISA Reagents and Primary Antibody Detection ELISA Kits |
Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA Western Accessories |
Catalog# |
Product Name. |
SIZE |
80012 |
High binding ELISA Strips plates (8 wellsx12 strips) |
10/pk |
80050 |
ELISA Plate Coating buffer concentrate (10X) |
50 ml |
80060 |
ELISA Plate Blocking Buffer concentrate (10X) BSA-based |
50 ml |
80062 |
ELISA Plate Blocking Buffer concentrate (10X) milk-based |
100 ml |
80070 |
Antibody and Conjugate Diluent for ELISA concentrate (10X) |
50 ml |
80080 |
Wash buffer concentrate (20X) for ELISA |
100 ml |
80081 |
Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X) |
100 ml |
80082 |
Tris Buffered Saline (TBS, pH 7.4) concentrate (10X) |
1 L |
80091 |
TMB substrate (1-component) for ELISA |
500 ml |
80100 |
Stop solution for TMB substrate (ELISA) concentrate (10X) |
50 ml |
80110 |
Single solution TMB substrate (membrane) solution for western blot |
100 mls |
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Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA Kits for the detection of Abs |
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Primary Antibody Detection ELISA Kits for Animals and Humans |
Catalog# |
Product Description |
Size |
80150 |
Mouse Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80155 |
Rat Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80160 |
Rabbit Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80165 |
Goat Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80166 |
Sheep Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80170 |
Human Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80171 |
G. Pig Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80175 |
Monkey Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80176 |
Chicken Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80180 |
Hamster Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80185 |
Bovine Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80186 |
Pig Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80187 |
Turkey Serum Antibody detection ELISA kit, Qualitative |
1 kit |
80188 |
Ferret Serum Antibody detection ELISA kit, Qualitative |
1 kit |
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Note: These kits provide all necessary reagents (coating, blocking buffer, primary antibody diluent, wash buffer concentrate, species specific secondary antibody-HRP Conjugate, and Single solution ready to use TMB, Stop solution) to perform animal specific serum antibody detection titration using user supplied coating antigen. Sufficient reagents for 1000 wells or 10-plates (100 ul reaction) or 2000 wells (20-plates @ 50 ul reaction). High binding ELISA plates #80012 can also be purchased. These kits are not designed to determine the serum IgG concn but detect antibodies to a given antigen. Please see IgG, IgA, IgM ELISA kits for measuring the levels of these proteins in serum |
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Negative Controls (Non-immune) Ig’s for ELISA, Western, and IHC |
Ig’s are collected and purified from a healthy serum pool of non-immunized animals. These Ig’s can be used as negative control in most immunoassays, including ELISA, Western, and IHC. Please see complete listing at the web site listed under “Primary Antibodies Category under Control IgG’s |
Species |
Catalog# |
Bovine IgG |
20001-1 |
Bovine IgM |
20001-2-1 |
Chicken IgG |
20010-1 |
Chicken IgM |
20010-2-1 |
Chicken IgY |
20111-1 |
Chicken Egg Yolk proteins |
20112 |
Chicken egg white proteins |
20113 |
Dog IgG |
20016-1 |
Dog IgG Fab |
20016-1-FAB |
Dog IgG F(ab')2 |
20016-1-
FAB2 |
Dog IgG (Fc) |
20016-1-FC |
Dog IgM |
20016-2-1 |
Donkey IgG |
20015-1 |
Duck IgY |
20114-1 |
Goat IgG |
20011-1
20011-25
20011-100 |
Goat IgG Fab |
20011-1-FAB |
Goat IgG F(ab')2 |
20011-1-
FAB2 |
Goat IgG (Fc) fragment |
20011-1-FC |
Goat IgM |
20011-2-1 |
Goose IgY |
20117-1 |
G. Pig IgG |
20004-1 |
G. Pig IgM |
20004-2-1 |
Hamster (Syrian) IgG |
20003-1 |
Hamster (Armenian) IgG |
20003-1AH |
Hamster IgM |
20003-2-1 |
Human IgG |
20007-1-1
20007-1-5 |
Human IgG Fab |
20007-1-FAB |
Human IgG F(ab')2 |
20007-1-
FAB2 |
Human IgG (Fc) |
20007-1-FC
20007-F1000 |
Human IgM |
20007-2
20007-2-5 |
Human IgM Fab mu |
20007-4 |
Human IgM Fc5u |
20007-2-
FC5M |
Human IgE (myeloma) |
20007-3 |
Human IgE(plasma) |
20007-3P |
Human IgA |
20007-5-1 |
Human IgA1 |
20007-6-100 |
Human IgA2 |
20007-7-100 |
Monkey IgG |
20012-1 |
Monkey IgM |
20013-1 |
Mouse IgG |
20008-1
20008-5
20008-100
20008-1-100 |
Mouse IgG1k |
20101-1 |
Mouse IgA |
20102-100 |
Mouse IgG Fab |
20008-1-FAB |
Mouse IgG F(ab')2 |
20008-1-
FAB2 |
Mouse IgG (Fc) |
20008-1-FC |
Mouse IgG1 |
20102-101 |
Mouse IgG2a |
20102-102 |
Mouse IgG2b |
20102-103 |
Mouse IgG3 |
20102-104 |
Mouse IgM |
20102-105
20008-2 |
Mouse IgM |
20102-105
20008-2 |
Mouse IgE |
20102-106 |
Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE set |
20102-SET |
Pig IgG |
20017-1 |
Pig IgG |
20017-2-1 |
Rabbit IgG |
20009-1
20009-25
20009-1-100 |
Rabbit IgG Fab |
20009-1-FAB |
Rabbit IgG F(ab')2 |
20009-1-
FAB2 |
Rabbit IgG (Fc) |
20009-1-FC |
Rabbit IgM |
20009-2-1
20039-1000 |
Rat IgG |
20005-1
20005-5 |
Rat IgG Fab |
20005-1-FAB |
Rat IgG F(ab')2 |
20005-1-
FAB2 |
Rat IgG (Fc) |
20005-1-FC |
Rat IgM |
20005-2-1 |
Sheep IgG |
20006-1 |
Sheep IgG (Fab2) |
30329 |
Sheep IgM |
20006-2 |
Sheep IgA |
20006-3 |
Turkey IgG |
20115-1 |
Turkey IgY |
20211-1 |
Quail IgG |
20311-1
20311-5 |
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Negative Control Sera (Non-immune) for ELISA, Western, and IHC |
Please see complete listing at the web site listed under “2 Antibodies & ELISA Reagents Category under Normal/Pre-immune (control) Sera |
Species |
Catalog# |
Baboon |
NBBS-1 & NBBS-5 |
Bovine |
NBOS-10 & NBOS-500 |
Chicken |
NCHS-10 & NCHS-500 |
Chimpanzee |
NCMS-1 & NCMS-5 |
Cat |
NCTS-1 & NCTS-5 |
Dog |
NDOS-1 & NDOS-5 |
Donkey |
NDKS-1 & NDKS-10 & NDKS-500 |
Duck |
NDUS-1 |
Ferret |
NFCS-1 & NFCS-5 |
Guinea Pig |
NGPS-25 & NGPS-5 |
Goat |
NGTS-1 & NGTS-500 |
Hamster (syr) |
NGHS-1& NGHS-5 |
Hams (Arm) |
NHUS-1 & NHUS-50 |
Monkey |
NMCS-1 (Cyano)
NMRS-1 (Rhesus)
NSQS-1 (Sq. monkey) |
Mouse |
NMOS-10 & NMOS-10 |
Pig/Swine |
NPIS-1 & NPIS-10 & NPIS-100 |
Pigeon |
NPNS-1 |
Rabbit |
NRBS-1 & NRBS-10 & NRBS-100 |
Rat |
NRTS-5 & NRTS-10 & NRTS-50 |
Sheep |
NSHS-10 & NSHS-100 |
Turkey |
NTUS-1 & |
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