General Purpose Western Blot Reagents

General Purpose Western Blot Reagents

Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA Western Accessories
Catalog# Product Name.  
80081 Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X) 100 ml
80082 Tris Buffered Saline (TBS, pH 7.4) concentrate (10X) 1 L
80215 NuGlo ECL Substrate Kit for Western blot, 400 mls 1 kit
80215-250 NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls 1 kit
80400-100 Bovine Serum Albumin (BSA, protease-free) for western (makes ~2-L buffer @5% BSA) 100 g
80401-250 Blotto for Western/ELISA (non-fat milk, makes ~25-L buffer @5%) 250 g
80402-500 Blotto 1 buffer (5% milk, 0.05% tween in TBS buffer) for Western 500 ml
80403-500 Blotto 2 buffer (1% milk, 1% BSA, 0.05% Tween in TBS for phosphoproteins) for WB 500 ml
ALP15-N-1 Alkaline phosphatase (AP), calf intestine, Conjugation grade (3000-6000 u/mg) 1 mg
ALP16-N-1 Alkaline phosphatase (AP), calf intestine, Mol Biol grade (3000-6000 u/mg) (Free of endonuclease, exonuclease and RNase activities) 1 mg
GO11-N-250 Glucose Oxidase (GO) enzyme (~200-300 u/mg) 250 mg
LMWM-12 Low range multi-color protein markers (6.5-45 kDa) for Western 250 ul
HMWM-11 High range multi-color protein markers (6.5-205 kDa) for Western 250 ul
HRP15-N Horse Radish Peroxidase (HRP) Enzyme, purified 1 mg
HRP16-N-500 Horse Radish Peroxidase (HRP) Enzyme (~200 u/mg) 500 mg
HRPS-100 Antibody/Protein-Peroxidase-Conjugate stabilizer solution 100 ml
PRTS-100 Coating Antibody or Protein stabilizer solution (10X) 100 ml
RIPA-50 RIPA lysis buffer kit for mammalian cell lysis (complete with protease inhibitor cocktail (PMSF, Protease inhibitors, Orthovanadate) 50 ml
SALL-500 StainAll dye for the visualization of proteins on membranes (transfer) 500 ml
TW-100110 Tween-20 (50%, convenient to dispense) for ELISA, Western, IP etc 500 ml
 

Western Blot Kits for Human & Animals

Catalog# Product Name.  
80200 Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate 1 kit
80201 Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate 1 kit
80202 Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate 1 kit
80203 Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate 1 kit
80204 Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate 1 kit
80205 Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate 1 kit
80206 Western blot Kit for G. pig Primary Antibodies, Chemilum. Substrate 1 kit
80207 Western blot Kit for Human Primary Antibodies, Chemilum. Substrate 1 kit
80208 Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate 1 kit
80209 Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate 1 kit
Notes: Items # 80202-80209 provide all necessary reagents (blocking buffer, primary antibody diluent, wash buffer concentrate, species specific secondary antibody-HRP Conjugate, and NuGlo ECL Substrate) to perform Western Blots. It has sufficient reagents to process 2000-4000 cm2 (10-20 mini blots) or 200-400 single lane strips.

Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under Western Blot Recycling kit
 

General Purpose ELISA Reagents and Primary Antibody Detection ELISA Kits

Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA Western Accessories
Catalog# Product Name. SIZE
80012 High binding ELISA Strips plates (8 wellsx12 strips) 10/pk
80050 ELISA Plate Coating buffer concentrate (10X) 50 ml
80060 ELISA Plate Blocking Buffer concentrate (10X) BSA-based 50 ml
80062 ELISA Plate Blocking Buffer concentrate (10X) milk-based 100 ml
80070 Antibody and Conjugate Diluent for ELISA concentrate (10X) 50 ml
80080 Wash buffer concentrate (20X) for ELISA 100 ml
80081 Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X) 100 ml
80082 Tris Buffered Saline (TBS, pH 7.4) concentrate (10X) 1 L
80091 TMB substrate (1-component) for ELISA 500 ml
80100 Stop solution for TMB substrate (ELISA) concentrate (10X) 50 ml
80110 Single solution TMB substrate (membrane) solution for western blot 100 mls
Please see complete listing at the web site listed under “2-antibodies & ELISA Reagents Category under ELISA Kits for the detection of Abs
 

Primary Antibody Detection ELISA Kits for Animals and Humans

Catalog# Product Description Size
80150 Mouse Serum Antibody detection ELISA kit, Qualitative 1 kit
80155 Rat Serum Antibody detection ELISA kit, Qualitative 1 kit
80160 Rabbit Serum Antibody detection ELISA kit, Qualitative 1 kit
80165 Goat Serum Antibody detection ELISA kit, Qualitative 1 kit
80166 Sheep Serum Antibody detection ELISA kit, Qualitative 1 kit
80170 Human Serum Antibody detection ELISA kit, Qualitative 1 kit
80171 G. Pig Serum Antibody detection ELISA kit, Qualitative 1 kit
80175 Monkey Serum Antibody detection ELISA kit, Qualitative 1 kit
80176 Chicken Serum Antibody detection ELISA kit, Qualitative 1 kit
80180 Hamster Serum Antibody detection ELISA kit, Qualitative 1 kit
80185 Bovine Serum Antibody detection ELISA kit, Qualitative 1 kit
80186 Pig Serum Antibody detection ELISA kit, Qualitative 1 kit
80187 Turkey Serum Antibody detection ELISA kit, Qualitative 1 kit
80188 Ferret Serum Antibody detection ELISA kit, Qualitative 1 kit
Note: These kits provide all necessary reagents (coating, blocking buffer, primary antibody diluent, wash buffer concentrate, species specific secondary antibody-HRP Conjugate, and Single solution ready to use TMB, Stop solution) to perform animal specific serum antibody detection titration using user supplied coating antigen. Sufficient reagents for 1000 wells or 10-plates (100 ul reaction) or 2000 wells (20-plates @ 50 ul reaction). High binding ELISA plates #80012 can also be purchased. These kits are not designed to determine the serum IgG concn but detect antibodies to a given antigen. Please see IgG, IgA, IgM ELISA kits for measuring the levels of these proteins in serum
 

Negative Controls (Non-immune) Ig’s for ELISA, Western, and IHC

Ig’s are collected and purified from a healthy serum pool of non-immunized animals. These Ig’s can be used as negative control in most immunoassays, including ELISA, Western, and IHC. Please see complete listing at the web site listed under “Primary Antibodies Category under Control IgG’s
Species Catalog#
Bovine IgG 20001-1
Bovine IgM 20001-2-1
Chicken IgG 20010-1
Chicken IgM 20010-2-1
Chicken IgY 20111-1
Chicken Egg Yolk proteins 20112
Chicken egg white proteins 20113
Dog IgG 20016-1
Dog IgG Fab 20016-1-FAB
Dog IgG F(ab')2 20016-1-
FAB2
Dog IgG (Fc) 20016-1-FC
Dog IgM 20016-2-1
Donkey IgG 20015-1
Duck IgY 20114-1
Goat IgG 20011-1
20011-25
20011-100
Goat IgG Fab 20011-1-FAB
Goat IgG F(ab')2 20011-1-
FAB2
Goat IgG (Fc) fragment 20011-1-FC
Goat IgM 20011-2-1
Goose IgY 20117-1
G. Pig IgG 20004-1
G. Pig IgM 20004-2-1
Hamster (Syrian) IgG 20003-1
Hamster (Armenian) IgG 20003-1AH
Hamster IgM 20003-2-1
Human IgG 20007-1-1
20007-1-5
Human IgG Fab 20007-1-FAB
Human IgG F(ab')2 20007-1-
FAB2
Human IgG (Fc) 20007-1-FC
20007-F1000
Human IgM 20007-2
20007-2-5
Human IgM Fab mu 20007-4
Human IgM Fc5u 20007-2-
FC5M
Human IgE (myeloma) 20007-3
Human IgE(plasma) 20007-3P
Human IgA 20007-5-1
Human IgA1 20007-6-100
Human IgA2 20007-7-100
Monkey IgG 20012-1
Monkey IgM 20013-1
Mouse IgG 20008-1
20008-5
20008-100
20008-1-100
Mouse IgG1k 20101-1
Mouse IgA 20102-100
Mouse IgG Fab 20008-1-FAB
Mouse IgG F(ab')2 20008-1-
FAB2
Mouse IgG (Fc) 20008-1-FC
Mouse IgG1 20102-101
Mouse IgG2a 20102-102
Mouse IgG2b 20102-103
Mouse IgG3 20102-104
Mouse IgM 20102-105
20008-2
Mouse IgM 20102-105
20008-2
Mouse IgE 20102-106
Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE set 20102-SET
Pig IgG 20017-1
Pig IgG 20017-2-1
Rabbit IgG 20009-1
20009-25
20009-1-100
Rabbit IgG Fab 20009-1-FAB
Rabbit IgG F(ab')2 20009-1-
FAB2
Rabbit IgG (Fc) 20009-1-FC
Rabbit IgM 20009-2-1
20039-1000
Rat IgG 20005-1
20005-5
Rat IgG Fab 20005-1-FAB
Rat IgG F(ab')2 20005-1-
FAB2
Rat IgG (Fc) 20005-1-FC
Rat IgM 20005-2-1
Sheep IgG 20006-1
Sheep IgG (Fab2) 30329
Sheep IgM 20006-2
Sheep IgA 20006-3
Turkey IgG 20115-1
Turkey IgY 20211-1
Quail IgG 20311-1
20311-5
 

Negative Control Sera (Non-immune) for ELISA, Western, and IHC

Please see complete listing at the web site listed under “2 Antibodies & ELISA Reagents Category under Normal/Pre-immune (control) Sera
Species Catalog#
Baboon NBBS-1 & NBBS-5
Bovine NBOS-10 & NBOS-500
Chicken NCHS-10 & NCHS-500
Chimpanzee NCMS-1 & NCMS-5
Cat NCTS-1 & NCTS-5
Dog NDOS-1 & NDOS-5
Donkey NDKS-1 & NDKS-10 & NDKS-500
Duck NDUS-1
Ferret NFCS-1 & NFCS-5
Guinea Pig NGPS-25 & NGPS-5
Goat NGTS-1 & NGTS-500
Hamster (syr) NGHS-1& NGHS-5
Hams (Arm) NHUS-1 & NHUS-50
Monkey NMCS-1 (Cyano)
NMRS-1 (Rhesus)
NSQS-1 (Sq. monkey)
Mouse NMOS-10 & NMOS-10
Pig/Swine NPIS-1 & NPIS-10 & NPIS-100
Pigeon NPNS-1
Rabbit NRBS-1 & NRBS-10 & NRBS-100
Rat NRTS-5 & NRTS-10 & NRTS-50
Sheep NSHS-10 & NSHS-100
Turkey NTUS-1 &